Monica Borucki

Contact information

Lawrence Livermore National Laboratory
7000 East Avenue, L-452
Livermore, CA 94550
phone: 925 424 4251
fax: 925 422 2282



Dr. Borucki is a Biomedical Scientist in the Biosciences and Biotechnology Division of LLNL. Her research focuses on the mechanisms of viral evolution and emergence, biosurveillence, and viral forensics. She previously served as Select Agent Manager for the LLNL Biodefense group and as a Biodefense Analyst for the LLNL Biodefense Knowledge Center. Before coming to LLNL, she served as a Research Geneticist at USDA-ARS and adjunct faculty at Washington State University where she investigated the epidemiology and virulence of food pathogen, Listeria monocytogenes. Her postdoctoral experience involved investigation of the ecology and genetics of South American hantaviruses at Univ. of Nevada, Reno. She received her Ph.D. from Colorado State University where she studied the evolution of La Crosse virus, a mosquito-borne virus that causes encephalitis. She received her M.S. at California Polytechnic State University San Luis Obispo investigating the revival and characterization of ancient Bacillus spores from bees entombed in amber. She has served as PI on competitive grants funded by DTRA, NIH, and internally funded LLNL projects. 

Recent publications

Devault, Alison M., Crystal Jaing, Shea Gardner, Teresita M. Porter, Jacob M. Enk, James Thissen, Jonathan Allen, Monica Borucki, Sharon N. DeWitte, Anna N. Dhody, Kevin McLoughin & Hendrik N. Poinar. 2014. Ancient pathogen DNA in archaeological samples detected with a Microbial Detection Array. SCIENTIFIC REPORTS 4 : 4245 DOI: 10.1038/srep04245

Chen-Harris, H., Borucki, M.K., Torres, C., Slezak, T.R., Allen, J.E., 2013. Ultra-deep mutant spectrum profiling: improving sequencing accuracy using overlapping read pairs. BMC genomics 14, 96.

Borucki, M.K., Allen, J.E., Chen-Harris, H., Zemla, A., Vanier, G., Mabery, S., Torres, C., Hullinger, P., Slezak, T., 2013a. The role of viral population diversity in adaptation of bovine coronavirus to new host environments. PloS one 8, e52752.

Borucki, M.K., Chen-Harris, H., Lao, V., Vanier, G., Wadford, D.A., Messenger, S., Allen, J.E., 2013b. Ultra-deep sequencing of intra-host rabies virus populations during cross-species transmission. PLoS neglected tropical diseases 7, e2555.

Ducey, T.F., Page, B., Usgaard, T., Borucki, M.K., Pupedis, K., Ward, T.J., 2007. A single-nucleotide-polymorphism-based multilocus genotyping assay for subtyping lineage I isolates of Listeria monocytogenes. Applied and environmental microbiology 73, 133-147.

Kim, S.H., Gorski, L., Reynolds, J., Orozco, E., Fielding, S., Park, Y.H., Borucki, M.K., 2006. Role of uvrA in the growth and survival of Listeria monocytogenes under UV radiation and acid and bile stress. Journal of food protection 69, 3031-3036.

Borucki, M.K., Gay, C.C., Reynolds, J., McElwain, K.L., Kim, S.H., Call, D.R., Knowles, D.P., 2005. Genetic diversity of Listeria monocytogenes strains from a high-prevalence dairy farm. Applied and environmental microbiology 71, 5893-5899.

Borucki, M.K., Reynolds, J., Call, D.R., Ward, T.J., Page, B., Kadushin, J., 2005. Suspension microarray with dendrimer signal amplification allows direct and high-throughput subtyping of Listeria monocytogenes from genomic DNA. Journal of clinical microbiology 43, 3255-3259.

Borucki, M.K., Kim, S.H., Call, D.R., Smole, S.C., Pagotto, F., 2004. Selective discrimination of Listeria monocytogenes epidemic strains by a mixed-genome DNA microarray compared to discrimination by pulsed-field gel electrophoresis, ribotyping, and multilocus sequence typing. Journal of clinical microbiology 42, 5270-5276.

Kim, S.H., Bakko, M.K., Knowles, D., Borucki, M.K., 2004. Oral inoculation of A/J mice for detection of invasiveness differences between Listeria monocytogenes epidemic and environmental strains. Infection and immunity 72, 4318-4321.

Ward, T.J., Gorski, L., Borucki, M.K., Mandrell, R.E., Hutchins, J., Pupedis, K., 2004. Intraspecific phylogeny and lineage group identification based on the prfA virulence gene cluster of Listeria monocytogenes. Journal of bacteriology 186, 4994-5002.

Borucki, M.K., Reynolds, J., Gay, C.C., McElwain, K.L., Kim, S.H., Knowles, D.P., Hu, J., 2004. Dairy farm reservoir of Listeria monocytogenes sporadic and epidemic strains. Journal of food protection 67, 2496-2499.

Call, D.R., Borucki, M.K., Besser, T.E., 2003. Mixed-genome microarrays reveal multiple serotype and lineage-specific differences among strains of Listeria monocytogenes. Journal of clinical microbiology 41, 632-639.

Borucki, M.K., Call, D.R., 2003. Listeria monocytogenes serotype identification by PCR. Journal of clinical microbiology 41, 5537-5540.

Borucki, M.K., Peppin, J.D., White, D., Loge, F., Call, D.R., 2003. Variation in biofilm formation among strains of Listeria monocytogenes. Applied and environmental microbiology 69, 7336-7342.

Borucki, M.K., Krug, M.J., Muraoka, W.T., Call, D.R., 2003. Discrimination among Listeria monocytogenes isolates using a mixed genome DNA microarray. Veterinary microbiology 92, 351-362.

Call, D.R., Borucki, M.K., Loge, F.J., 2003. Detection of bacterial pathogens in environmental samples using DNA microarrays. Journal of microbiological methods 53, 235-243.

Palumbo, J.D., Borucki, M.K., Mandrell, R.E., Gorski, L., 2003. Serotyping of Listeria monocytogenes by enzyme-linked immunosorbent assay and identification of mixed-serotype cultures by colony immunoblotting. Journal of clinical microbiology 41, 564-571.

Meissner, J.D., Rowe, J.E., Borucki, M.K., St Jeor, S.C., 2002. Complete nucleotide sequence of a Chilean hantavirus. Virus research 89, 131-143.

Borucki, M.K., Kempf, B.J., Blitvich, B.J., Blair, C.D., Beaty, B.J., 2002. La Crosse virus: replication in vertebrate and invertebrate hosts. Microbes and infection / Institut Pasteur 4, 341-350.

Borucki, M.K., Kempf, B.J., Blair, C.D., Beaty, B.J., 2001. The effect of mosquito passage on the La Crosse virus genotype. The Journal of general virology 82, 2919-2926.

Borucki, M.K., Boone, J.D., Rowe, J.E., Bohlman, M.C., Kuhn, E.A., DeBaca, R., St Jeor, S.C., 2000. Role of maternal antibody in natural infection of Peromyscus maniculatus with Sin Nombre virus. Journal of virology 74, 2426-2429.

Borucki, M.K., Chandler, L.J., Parker, B.M., Blair, C.D., Beaty, B.J., 1999. Bunyavirus superinfection and segment reassortment in transovarially infected mosquitoes. The Journal of general virology 80 ( Pt 12), 3173-3179.

Chandler, L.J., Borucki, M.K., Dobie, D.K., Wasieloski, L.P., Thompson, W.H., Gundersen, C.B., Case, K., Beaty, B.J., 1998. Characterization of La Crosse virus RNA in autopsied central nervous system tissues. Journal of clinical microbiology 36, 3332-3336.

Cano, R.J., Borucki, M.K., 1995. Revival and identification of bacterial spores in 25- to 40-million-year-old Dominican amber. Science 268, 1060-1064.

Cano, R.J., Borucki, M.K., Higby-Schweitzer, M., Poinar, H.N., Poinar, G.O., Jr., Pollard, K.J., 1994. Bacillus DNA in fossil bees: an ancient symbiosis? Applied and environmental microbiology 60, 2164-2167.